6W2G
Crystal Structure of Y188G Variant of the Internal UBA Domain of HHR23A in Monoclinic Unit Cell
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.490, 40.080, 32.893 |
Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
Resolution | 32.880 - 1.100 |
R-factor | 0.1399 |
Rwork | 0.139 |
R-free | 0.15270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P43 UBA-1 Y188G Structure Solved by Sulfur SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.036 |
Data reduction software | XDS (Mar 15,2019) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (1.17.1-3660) |
Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.880 | 1.139 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.017 | 0.079 |
Rmeas | 0.019 | 0.088 |
Rpim | 0.007 | 0.040 |
Number of reflections | 29568 | 2513 |
<I/σ(I)> | 56.13 | 16.63 |
Completeness [%] | 94.8 | 81.04 |
Redundancy | 6.2 | 4.7 |
CC(1/2) | 1.000 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 0.1M Phosphate-citrate pH 4.2, 40%(v/v) Ethylene glycol |