6W1K

Crystal structure of the hydroxyglutarate synthase in complex with 2-oxoadipate from Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	92.300, 98.200, 203.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.790 - 1.850
R-factor	0.1659
Rwork	0.165
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lho
RMSD bond length	0.011
RMSD bond angle	1.086
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	1.910
High resolution limit [Å]	1.850	1.850
Number of reflections	156493	15364
<I/σ(I)>	11.5
Completeness [%]	98.9
Redundancy	7.3
CC(1/2)	0.998	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	0.01 M Magnesium Chloride, 0.05 M MES pH 5.6, 1.8 M Lithium Sulfate