6W1E
Crystal structure of Streptococcus thermophilus SHP pheromone receptor Rgg3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 110.360, 110.360, 54.933 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.787 - 2.202 |
R-factor | 0.2695 |
Rwork | 0.265 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yv6 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.081 | 0.051 | 0.471 |
Rmeas | 0.086 | 0.054 | 0.503 |
Rpim | 0.028 | 0.018 | 0.172 |
Number of reflections | 19396 | 1059 | 826 |
<I/σ(I)> | 8.5 | ||
Completeness [%] | 98.0 | 99.4 | 86 |
Redundancy | 9.4 | 9.1 | 7.5 |
CC(1/2) | 0.998 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.2 | 298 | 100 mM sodium citrate, 300 mM Na/K tartrate, and 1.4 M ammonium sulfate |