6W0H

Closed-gate KcsA soaked in 5mM KCl/5mM BaCl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-10-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	I 4
Unit cell lengths	155.849, 155.849, 75.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.012 - 2.600
R-factor	0.1965
Rwork	0.194
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.012	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	28030	3403
<I/σ(I)>	5.9
Completeness [%]	99.9
Redundancy	9
CC(1/2)	0.550	0.210

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5