6W0D
Open-gate KcsA soaked in 5 mM BaCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 156.912, 156.912, 73.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.477 - 3.639 |
| R-factor | 0.1933 |
| Rwork | 0.190 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.477 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.250 | |
| Number of reflections | 10018 | 2204 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 97.9 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.990 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5 |
