6W0A

Open-gate KcsA soaked in 1 mM BaCl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	98
Detector technology	PIXEL
Collection date	2017-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	156.569, 156.569, 73.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.826 - 3.237
R-factor	0.1826
Rwork	0.179
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.011
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.826	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.180
Number of reflections	14339	2831
<I/σ(I)>	8.5
Completeness [%]	99.1
Redundancy	6
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 % PEG 400, 50 mM Magnesium Acetate, 50 mM Sodium Acetate, pH 5.5