6W08
Crystal Structure of Motility Associated Killing Factor E from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-05 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.209, 45.134, 143.950 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 49.490 - 1.750 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | the same protein structure from SAD phasing with a lower resolution. |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.533 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.105 | 1.053 |
| Number of reflections | 74404 | 3667 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 98.0 | 97.5 |
| Redundancy | 4.1 | 3.7 |
| CC(1/2) | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Potassium thiocyanate 0.1M Bis Tris propane pH 6.5 20% w/v PEG 3350 |
