6W03

Crystal Structure of HIV-1 BG505 DS-SOSIP.3mut Prefusion Env Trimer in Complex with Human Antibodies 3H109L and 35O22 at 3.3 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-04
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	131.390, 131.390, 315.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.800 - 2.400
R-factor	0.2325
Rwork	0.230
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mtj
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.370	5.170	2.400
Rmerge	0.149	0.093	0.426
Rmeas	0.174	0.110	0.542
Rpim	0.087	0.057	0.331
Number of reflections	50660	11780	269
<I/σ(I)>	5.8
Completeness [%]	42.3	97.5	2.3
Redundancy	3.3	4.1	1.1
CC(1/2)		0.992	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	75 mM imidazole pH6.5, 150 mM LiSO4, 3.8% PEG MPD and 6.2% PEG 3,350