6VZ0

C-terminal domain of mouse surfactant protein B crystallized at high pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	38.396, 58.360, 64.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.330 - 1.750
R-factor	0.179
Rwork	0.177
R-free	0.21220
Structure solution method	AB INITIO PHASING
RMSD bond length	0.013
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	XDS
Phasing software	Arcimboldo
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.330	1.817
High resolution limit [Å]	1.750	1.750
Rmerge	0.091
Rmeas	0.099
Rpim	0.039
Number of reflections	14990	1392
<I/σ(I)>	16.52
Completeness [%]	98.9	95.21
Redundancy	6.4
CC(1/2)	0.998	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	100 mM Tris pH 8.5, 200 mM lithium sulfate, 1260 mM ammonium sulfate