6VYN
N-terminal domain of mouse surfactant protein B with bound lipid, wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.707, 108.156, 114.741 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.700 - 2.200 |
| R-factor | 0.2243 |
| Rwork | 0.224 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.333 |
| Data reduction software | XDS |
| Data scaling software | PHENIX |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.700 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 112655 | 7105 |
| <I/σ(I)> | 24.77 | 0.42 |
| Completeness [%] | 95.2 | 61.16 |
| Redundancy | 16 | 6.6 |
| CC(1/2) | 0.994 | 0.236 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293.15 | 17% PEG3350, 0.1M citrate buffer pH 5.4, 0.15 M NaCl |
