6VYC

Crystal structure of WD-repeat domain of human WDR91

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-10
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 21 2 21
Unit cell lengths	77.004, 84.047, 110.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.070 - 2.100
R-factor	0.2227
Rwork	0.221
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ow8
RMSD bond length	0.005
RMSD bond angle	1.348
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.111	46.070	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.091	0.027	0.906
Rmeas	0.098	0.030	0.966
Rpim	0.034	0.011	0.331
Total number of observations		4292	27080
Number of reflections	41340	646	3269
<I/σ(I)>	16.8	51.7	2.8
Completeness [%]	97.6	99.1	95.6
Redundancy	8	6.6	8.3
CC(1/2)	0.999	0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	12% PEG20000, 0.1 M MES