6VY6

Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV-26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-26
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 2 21 21
Unit cell lengths	70.807, 79.767, 177.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.347 - 2.600
R-factor	0.199
Rwork	0.197
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x9m
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.460	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.118	0.757
Number of reflections	31674	4550
<I/σ(I)>	16.8
Completeness [%]	99.9
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% MPD, 0.1 imidazole, pH 6.5, 0.2 M ammonium sulfate, 10% PEG3350