6VY5

Crystal structure of Nipah receptor binding protein head domain in complex with human neutralizing antibody HENV-26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 32 2 1
Unit cell lengths	186.701, 186.701, 81.312
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.924 - 3.400
R-factor	0.2105
Rwork	0.208
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vwd
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.141	0.514
Number of reflections	22696	3283
<I/σ(I)>	7.2
Completeness [%]	100.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.0 M sodium malonate, pH 7.0, 0.1 M Bis-Tris propane, pH 7.0