6VVN
F6 fused 4-OT wild type asymmetric trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.196, 69.448, 93.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.870 - 1.390 |
| Rwork | 0.168 |
| R-free | 0.18650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 (1) |
| Data scaling software | HKL-2000 (1) |
| Phasing software | MOLREP (1) |
| Refinement software | PHENIX (v1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.870 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Number of reflections | 62808 | 6174 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298.15 | 8% ethylene glycol, 20-23% PEG10000 |
