6VVI

Arabidopsis thaliana dihydrodipicolinate synthase isoform 1 (DHDPS1)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 21 21 21
Unit cell lengths	95.468, 98.100, 176.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.098 - 2.145
Rwork	0.172
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dpp
RMSD bond length	0.006
RMSD bond angle	1.404
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.200
High resolution limit [Å]	2.140	2.140
Number of reflections	90994	4163
<I/σ(I)>	11.5
Completeness [%]	99.4
Redundancy	6.7
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.5), 0.1 M NaCl, and 1.4 M (NH4)2SO4