6VV4
Scabin (V109G) toxin from Streptomyces scabies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.910, 61.070, 37.970 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 37.403 - 1.700 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5daz |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.403 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.039 | 1.178 |
| Rmeas | 0.046 | |
| Rpim | 0.024 | 0.812 |
| Number of reflections | 21470 | 1917 |
| <I/σ(I)> | 19.12 | 0.95 |
| Completeness [%] | 98.1 | 88.14 |
| Redundancy | 3.7 | 2.9 |
| CC(1/2) | 0.999 | 0.437 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM potassium chloride, 50 mM sodium cacodylate trihydrate, pH 6.0, 16% PEG1000, 0.5 mM spermine |
