6VU2
M1214_N1 Fab structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.920103 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.692, 66.888, 70.176 |
| Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.190 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tsc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS (1.16_3549) |
| Data scaling software | XDS (1.16_3549) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.350 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.059 | 0.407 |
| Rmeas | 0.075 | 0.525 |
| Rpim | 0.045 | 0.326 |
| Number of reflections | 21766 | 1621 |
| <I/σ(I)> | 10.6 | 1.91 |
| Completeness [%] | 95.2 | 72 |
| Redundancy | 2.4 | 1.8 |
| CC(1/2) | 0.996 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 28% PEG8000, 1.6M calcium acetate and 20% glycerol |
