6VTH

p53-specific T cell receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	65.615, 84.955, 88.749
Unit cell angles	90.00, 93.61, 90.00

Refinement procedure

Resolution	20.000 - 2.360
R-factor	0.214
Rwork	0.211
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sjv 2ak4
RMSD bond length	0.006
RMSD bond angle	1.373
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.440
High resolution limit [Å]	2.360	2.360
Rmerge	0.096	0.263
Number of reflections	36665	3669
<I/σ(I)>	13.9
Completeness [%]	91.1
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295	18% (w/v) PEG 2000, 0.2 M AmSO4, 0.1 M MES