6VRO
The structure of the PP2A B56 subunit AIM1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 |
| Unit cell lengths | 111.044, 111.044, 108.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.880 - 2.450 |
| R-factor | 0.2211 |
| Rwork | 0.220 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k6s |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.14) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.260 | 39.260 | 2.550 |
| High resolution limit [Å] | 2.450 | 8.830 | 2.450 |
| Rmerge | 0.098 | 0.022 | 1.888 |
| Rmeas | 0.106 | 0.024 | 2.036 |
| Rpim | 0.040 | 0.009 | 0.757 |
| Total number of observations | 3577 | 19056 | |
| Number of reflections | 24266 | 531 | 2711 |
| <I/σ(I)> | 12.4 | 56.5 | 1.2 |
| Completeness [%] | 99.8 | 98 | 99.4 |
| Redundancy | 7 | 6.7 | 7 |
| CC(1/2) | 0.999 | 1.000 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris, pH 8.0, 9% PEG8000, 0.9 M lithium chloride |
