6VR4
Virion-packaged DNA-dependent RNA polymerase of crAss-like phage phi14:2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 266.441, 297.181, 92.015 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.930 - 3.500 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.23940 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.930 | 31.930 | 3.710 |
High resolution limit [Å] | 3.500 | 10.300 | 3.500 |
Rmerge | 0.176 | 0.031 | 0.942 |
Rmeas | 0.189 | 0.033 | 1.007 |
Total number of observations | 1401490 | ||
Number of reflections | 177687 | 6928 | 28518 |
<I/σ(I)> | 11.9 | 50.4 | 2.25 |
Completeness [%] | 99.7 | 98.9 | 99.4 |
Redundancy | 7.887 | 7.468 | 7.897 |
CC(1/2) | 0.996 | 0.999 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris-HCl, pH 8.5, 200 mM sodium acetate, 11% PEG4000, 2 mM TCEP |