6VR4

Virion-packaged DNA-dependent RNA polymerase of crAss-like phage phi14:2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-01
Detector	RAYONIX MX300HE
Wavelength(s)	0.97872
Spacegroup name	P 21 21 2
Unit cell lengths	266.441, 297.181, 92.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.930 - 3.500
R-factor	0.1943
Rwork	0.192
R-free	0.23940
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.930	31.930	3.710
High resolution limit [Å]	3.500	10.300	3.500
Rmerge	0.176	0.031	0.942
Rmeas	0.189	0.033	1.007
Total number of observations	1401490
Number of reflections	177687	6928	28518
<I/σ(I)>	11.9	50.4	2.25
Completeness [%]	99.7	98.9	99.4
Redundancy	7.887	7.468	7.897
CC(1/2)	0.996	0.999	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100 mM Tris-HCl, pH 8.5, 200 mM sodium acetate, 11% PEG4000, 2 mM TCEP