6VPE
Crystal structure of transcriptional regulator from bacteriophage 186
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.4586 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.860, 46.140, 38.310 |
| Unit cell angles | 90.00, 99.42, 90.00 |
Refinement procedure
| Resolution | 44.447 - 2.211 |
| R-factor | 0.233 |
| Rwork | 0.227 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vli |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.653 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.450 | 44.450 | 2.280 |
| High resolution limit [Å] | 2.210 | 9.120 | 2.210 |
| Rmerge | 0.144 | 0.096 | 0.854 |
| Rmeas | 0.155 | 0.105 | 0.926 |
| Rpim | 0.058 | 0.040 | 0.352 |
| Total number of observations | 99947 | 1502 | 8094 |
| Number of reflections | 14298 | 232 | 1197 |
| <I/σ(I)> | 11.2 | 21 | 3.4 |
| Completeness [%] | 97.8 | 99.3 | 95.5 |
| Redundancy | 7 | 6.5 | 6.8 |
| CC(1/2) | 0.995 | 0.992 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 2 M ammonium sulfate, 0.1 M Bis-Tris:HCl, pH 5.5 |
