6VP5
Ethylene forming enzyme (EFE) D191E variant in complex with Fe(II), L-arginine, and 2OG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.542, 78.500, 195.492 |
| Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
| Resolution | 49.520 - 1.970 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lsq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.634 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.15.2_3472) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.520 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rpim | 0.040 | 0.280 |
| Number of reflections | 105982 | 9842 |
| <I/σ(I)> | 16.65 | |
| Completeness [%] | 99.2 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris propane, 0.1-0.2 M sodium chloride, 24-30% PEG6000 |
