6VOS
Crystal structure of macaque anti-HIV-1 antibody RM20J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03322 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 111.260, 111.260, 141.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.867 - 2.299 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vor |
RMSD bond length | 0.007 |
RMSD bond angle | 0.949 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.299 | 2.300 |
Number of reflections | 40057 | 1946 |
<I/σ(I)> | 28 | |
Completeness [%] | 100.0 | |
Redundancy | 17.5 | |
CC(1/2) | 0.897 | 0.441 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | The RM20J Fab was crystallized from a solution containing 10 mg/mL protein in 1X TBS with a well solution containing 0.1M MES, pH 6.0, 5% PEG3000 and 40% PEG400, with no cryoprotectant supplemented. |