6VOS

Crystal structure of macaque anti-HIV-1 antibody RM20J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03322
Spacegroup name	P 43 21 2
Unit cell lengths	111.260, 111.260, 141.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.867 - 2.299
R-factor	0.1827
Rwork	0.181
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vor
RMSD bond length	0.007
RMSD bond angle	0.949
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.299	2.300
Number of reflections	40057	1946
<I/σ(I)>	28
Completeness [%]	100.0
Redundancy	17.5
CC(1/2)	0.897	0.441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	The RM20J Fab was crystallized from a solution containing 10 mg/mL protein in 1X TBS with a well solution containing 0.1M MES, pH 6.0, 5% PEG3000 and 40% PEG400, with no cryoprotectant supplemented.