6VOP
Crystal structure of YgbL, a putative aldolase/epimerase/decarboxylase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-01-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 174.796, 174.796, 174.796 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.480 - 2.650 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2opi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.471 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.272 | 1.146 |
| Rpim | 0.049 | 0.389 |
| Number of reflections | 7074 | 353 |
| <I/σ(I)> | 17.48 | 2.08 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 29.6 | 9 |
| CC(1/2) | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1M Na Acetate pH 4.5, 3 M Na Chloride |
