6VOP

Crystal structure of YgbL, a putative aldolase/epimerase/decarboxylase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-01-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	174.796, 174.796, 174.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.480 - 2.650
R-factor	0.1914
Rwork	0.189
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2opi
RMSD bond length	0.002
RMSD bond angle	0.471
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.272	1.146
Rpim	0.049	0.389
Number of reflections	7074	353
<I/σ(I)>	17.48	2.08
Completeness [%]	100.0	100
Redundancy	29.6	9
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.1M Na Acetate pH 4.5, 3 M Na Chloride