6VN3
USP7 IN COMPLEX WITH LIGAND COMPOUND 23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.510, 65.834, 85.077 |
| Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
| Resolution | 48.047 - 2.730 |
| R-factor | 0.2248 |
| Rwork | 0.222 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.800 | 84.800 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.068 | 0.444 |
| Rmeas | 0.084 | 0.052 |
| Total number of observations | 52057 | |
| Number of reflections | 20126 | 81 |
| <I/σ(I)> | 10.27 | 29.9 |
| Completeness [%] | 96.4 | 97.2 |
| Redundancy | 2.6 | 2.6 |
| CC(1/2) | 0.995 | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | NULL |
