6VMW
Crystal structure of the F316A mutant of GoxA soaked with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.607, 91.883, 178.786 |
| Unit cell angles | 90.00, 91.37, 90.00 |
Refinement procedure
| Resolution | 45.941 - 1.990 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3byw |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.210 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.100 | 0.560 |
| Rmeas | 0.117 | 0.669 |
| Rpim | 0.061 | 0.359 |
| Number of reflections | 240912 | 11746 |
| <I/σ(I)> | 9 | 2.2 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.994 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.5 | 293 | Protein at 10 mg/mL was combined in a 1:1 ratio with precipitant containing 19% PEG 3350, 0.1 M citrate pH 5.5 |
