6VMV
Crystal structure of the H767A mutant of GoxA soaked with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.965, 93.081, 187.642 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 48.197 - 2.200 |
| R-factor | 0.1873 |
| Rwork | 0.187 |
| R-free | 0.23850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6byw |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.513 |
| Rmeas | 0.129 | 0.609 |
| Rpim | 0.067 | 0.322 |
| Number of reflections | 189420 | 9379 |
| <I/σ(I)> | 5.9 | 2.1 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.993 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | Protein at 10 mg/mL was combined at a 1:1 ratio with precipitation solution containing 23% PEG 3350, 0.1 M HEPES pH 7.5, 0.1 M ammonium sulfate |
