6VM6

Structure of Acinetobacter baumannii Cap4 SAVED/CARF-domain containing receptor with the cyclic trinucleotide 2'3'3'-cAAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	106.577, 111.457, 164.830
Unit cell angles	90.00, 100.21, 90.00

Refinement procedure

Resolution	49.210 - 2.100
R-factor	0.1857
Rwork	0.185
R-free	0.21920
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SOLVE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.042	0.811
Number of reflections	217277	10452
<I/σ(I)>	12.4
Completeness [%]	98.3
Redundancy	7.2
CC(1/2)	0.998	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	10% PEG-3350, 200 mM lithium sulfate, 100 mM imidazole pH 8.0