6VLI
Crystal structure of transcriptional regulator from bacteriophage 186
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.470, 53.510, 106.350 |
| Unit cell angles | 90.00, 100.88, 90.00 |
Refinement procedure
| Resolution | 54.473 - 2.100 |
| R-factor | 0.223080930671 |
| Rwork | 0.221 |
| R-free | 0.26068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD model from previous experiments |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.483 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2689) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.473 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.248 | 1.492 |
| Rmeas | 0.270 | 1.622 |
| Rpim | 0.105 | 0.627 |
| Number of reflections | 35935 | 2958 |
| <I/σ(I)> | 5 | 1.3 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.5 | 6.6 |
| CC(1/2) | 0.986 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 8% Tascimate pH 8.0, 20% PEG 3350 |
