6VKJ

Crystal structure of the G domain of human guanylate-binding protein 2 (hGBP2) in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-07-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920126
Spacegroup name	P 41 21 2
Unit cell lengths	87.951, 87.951, 105.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.168 - 2.105
R-factor	0.1845
Rwork	0.181
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2B92:A
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	2.181
High resolution limit [Å]	2.105	2.105
Rmeas	0.255
Rpim	0.081	0.603
Number of reflections	45429	4533
<I/σ(I)>	17.8
Completeness [%]	99.3	100
Redundancy	9	5.5
CC(1/2)	0.985	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.04M KH2PO4, 12% PEG 8000, 18% glycerol