6VK4

Crystal Structure of Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase and Regulatory Component Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	102.861, 105.207, 300.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.590 - 2.350
R-factor	0.1919
Rwork	0.190
R-free	0.22780
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.690
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20rc2_4400)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.860	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.147
Rpim		1.332
Number of reflections	131580	6698
<I/σ(I)>	5.7
Completeness [%]	98.4
Redundancy	4.3
CC(1/2)	0.993	0.287

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tacsimate and PEG3350