6VJV

Crystal structure of the Prochlorococcus phage (myovirus P-SSM2) ferredoxin at 1.6 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	52.619, 31.446, 61.288
Unit cell angles	90.00, 92.48, 90.00

Refinement procedure

Resolution	39.060 - 1.590
R-factor	0.1799
Rwork	0.178
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h57
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.570	52.570	1.617
High resolution limit [Å]	1.589	4.314	1.589
Rmerge	0.120	0.063	1.881
Rmeas	0.131	0.069	2.107
Rpim	0.050	0.027	0.914
Number of reflections	27020	1457	1178
<I/σ(I)>	8.9	21.5	1
Completeness [%]	98.5	99.8	86.1
Redundancy	6.6	6.3	5.1
CC(1/2)	0.996	0.995	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	10% (w/v) PEG 3000, 200 mM zinc acetate, and 100 mM sodium acetate/acetic acid pH 4.5