6VJL

Streptavidin mutant M112 (G26C/A46C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	I 41 2 2
Unit cell lengths	57.772, 57.772, 184.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.350 - 1.300
Rwork	0.190
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swe
RMSD bond length	0.007
RMSD bond angle	1.336
Data reduction software	XDS (20161205)
Data scaling software	XSCALE (20161205)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0135 2015/10/01)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.330
High resolution limit [Å]	1.300	1.300
Rmerge	0.073	0.010
Rmeas	0.076	1.110
Number of reflections	39166	2809
<I/σ(I)>	19.6
Completeness [%]	99.9	99.2
Redundancy	11	5.6
CC(1/2)	1.000	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294.15	28% PEG 4000, 0.15 M ammonium sulfate, 50 mM Bis-Tris