6VJF
The P-Loop K to A mutation of C. therm Vps1 GTPase-BSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 1998-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91299 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.873, 119.032, 84.576 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 80.380 - 2.472 |
| R-factor | 0.19093 |
| Rwork | 0.189 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6def |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.966 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.380 | 2.540 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.063 | 0.415 |
| Rmeas | 0.067 | 0.445 |
| Rpim | 0.024 | 0.157 |
| Number of reflections | 56551 | 4608 |
| <I/σ(I)> | 17.3 | 5.3 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 7.4 | 7.8 |
| CC(1/2) | 0.998 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 91 mM HEPES pH 8.1 16.7 % PEG 3,350 182 mM Sodium Acetate 3% PROPAN-2-OL |
