6VJ0

Crystal structure of a chitin-binding protein from Moringa oleifera seeds (Mo-CBP4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.450
Spacegroup name	I 41 2 2
Unit cell lengths	107.052, 107.052, 42.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.870 - 1.900
Rwork	0.198
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dom
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.870	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.073	1.688
Rpim	0.021	0.485
Number of reflections	10152	641
<I/σ(I)>	32	2.3
Completeness [%]	100.0	100
Redundancy	24.6	3.5
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6