6VJ0
Crystal structure of a chitin-binding protein from Moringa oleifera seeds (Mo-CBP4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.450 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 107.052, 107.052, 42.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.870 - 1.900 |
| Rwork | 0.198 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dom |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.870 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 1.688 |
| Rpim | 0.021 | 0.485 |
| Number of reflections | 10152 | 641 |
| <I/σ(I)> | 32 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.6 | 3.5 |
| CC(1/2) | 1.000 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.0 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6 |
