6VIS
The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:V62E Variant of HCRBPII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-27 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.123, 101.031, 109.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.130 - 2.790 |
| R-factor | 0.2386 |
| Rwork | 0.232 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.888 |
| High resolution limit [Å] | 2.788 | 2.788 |
| Rmerge | 0.148 | 0.819 |
| Rmeas | 0.160 | 0.886 |
| Number of reflections | 10793 | 1015 |
| <I/σ(I)> | 17.14 | 2.11 |
| Completeness [%] | 99.2 | 93.81 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 4000, Sodium acetate, ammonium acetate |
