6VIO

Crystal structure of eYFP His148Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	56.651, 56.772, 148.193
Unit cell angles	85.12, 85.26, 70.48

Refinement procedure

Resolution	37.380 - 3.600
R-factor	0.245
Rwork	0.237
R-free	0.31010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v3d
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.660
High resolution limit [Å]	3.600	9.750	3.600
Rmerge	0.161	0.056	0.853
Rmeas	0.207	0.075	1.062
Rpim	0.128	0.050	0.622
Total number of observations	40830
Number of reflections	18449	816	944
<I/σ(I)>	4.4
Completeness [%]	91.4	81	97.9
Redundancy	2.2	2.1	2.4
CC(1/2)		0.992	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.75 M Na/K phosphate pH 6.9