6VII

Crystal structure of mouse RABL3 in complex with GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.506, 81.175, 109.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.992 - 2.000
R-factor	0.1957
Rwork	0.194
R-free	0.22830
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.992	5.430	2.000
Rmerge	0.054	0.035	2.354
Rmeas	0.056	0.037	2.483
Rpim	0.015	0.011	0.767
Number of reflections	36727	2007	1778
<I/σ(I)>	8.6
Completeness [%]	99.7	99.7	98.1
Redundancy	12.8	12.4	9
CC(1/2)		0.997	0.437

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.6 M Ammonium phosphate monobasic