6VHF

Crystal structure of RbBP5 interacting domain of Cfp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	67.266, 71.614, 78.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.029 - 2.311
R-factor	0.2354
Rwork	0.233
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	preliminary incomplete model
RMSD bond length	0.008
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.980	2.440
High resolution limit [Å]	2.310	2.310
Number of reflections	8459	799
<I/σ(I)>	15
Completeness [%]	99.2
Redundancy	13.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.2 M NH4Cl, and 22% (w/v) PEG 3350