6VGV
Crystal structure of VidaL intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-30 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.122, 40.827, 36.188 |
| Unit cell angles | 90.00, 103.45, 90.00 |
Refinement procedure
| Resolution | 26.950 - 1.650 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | VidaL SeMet structure |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.796 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.950 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.063 | 0.323 |
| Rpim | 0.023 | 0.185 |
| Number of reflections | 17134 | 2567 |
| <I/σ(I)> | 4 | 3.5 |
| Completeness [%] | 98.9 | 94.6 |
| Redundancy | 5.9 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate (pH 4.6) with 2 M ammonium sulfate |
