6VFU

Crystal structure of human protocadherin 19 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	108.971, 108.971, 309.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 3.500
R-factor	0.2661
Rwork	0.265
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfq
RMSD bond length	0.009
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.710
High resolution limit [Å]	3.500	3.500
Rmerge	0.140	2.500
Rmeas	0.150	2.600
Rpim	0.047	0.814
Number of reflections	27770	4378
<I/σ(I)>	9.4	1.1
Completeness [%]	99.9	99.9
Redundancy	9.8	10.4
CC(1/2)	1.000	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	7%(w/v) PEG 4000 0.2M NaCl 0.05M MES pH6.5 15% (v/v) 2R,3R-butanediol added as cryoprotectant