6VFQ

Crystal structure of monomeric human protocadherin 10 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	26.373, 78.910, 238.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.300
R-factor	0.1906
Rwork	0.188
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dzv
RMSD bond length	0.014
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.890
Rmeas	0.110	1.000
Rpim	0.044	0.440
Number of reflections	23366	2283
<I/σ(I)>	11	1.6
Completeness [%]	99.7	98.8
Redundancy	6.1	4.7
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	296	14% PEG 400 (v/v), 0.1M sodium acetate pH 4.6, 0.1M CaCl2, with 30% ethylene glycol (v/v) added as cryoprotectant