6VFC

DNA Polymerase Mu, 8-oxorGTP:Ct Product State Ternary Complex, 50 mM Mn2+ (2160 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.333, 62.440, 119.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.549 - 1.590
R-factor	0.1734
Rwork	0.172
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.620
High resolution limit [Å]	1.589	4.320	1.590
Rmerge	0.050	0.025	0.952
Rmeas	0.056	0.027	1.050
Rpim	0.023	0.011	0.433
Number of reflections	58898	3249	2946
<I/σ(I)>	12.7
Completeness [%]	95.8	97.9	97.2
Redundancy	5.6	5.5	5.6
CC(1/2)		0.999	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000