6VF8

DNA Polymerase Mu, 8-oxorGTP:Ct Pre-Catalytic Ternary Complex, 20 mM Ca2+ (120 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.101, 62.271, 118.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.944 - 1.700
R-factor	0.1695
Rwork	0.168
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.699	4.610	1.700
Rmerge	0.111	0.080	0.763
Rmeas	0.120	0.088	0.840
Rpim	0.046	0.035	0.346
Number of reflections	49817	2699	2482
<I/σ(I)>	8.1
Completeness [%]	99.9	99.7	100
Redundancy	6.6	6.4	5.7
CC(1/2)		0.986	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000