6VF0

DNA Polymerase Mu, 8-oxorGTP:At Reaction State Ternary Complex, 50 mM Mg2+ (30 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.982, 68.437, 110.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.956 - 1.580
R-factor	0.1646
Rwork	0.164
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.574	4.290	1.580
Rmerge	0.105	0.093	0.971
Rmeas	0.114	0.102	1.056
Rpim	0.044	0.040	0.410
Number of reflections	63540	3403	3118
<I/σ(I)>	10.3
Completeness [%]	99.8	99.1	99.6
Redundancy	6.6	6.3	6.2
CC(1/2)		0.983	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000