6VEA
Structure of the Glutamate-Like Receptor GLR3.2 ligand-binding domain in complex with Glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.389, 64.370, 75.933 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.230 - 1.580 |
| R-factor | 0.1588 |
| Rwork | 0.158 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ve8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.390 | 47.390 | 1.610 |
| High resolution limit [Å] | 1.580 | 8.650 | 1.580 |
| Rmerge | 0.062 | 0.029 | 0.612 |
| Rmeas | 0.069 | 0.033 | 0.713 |
| Rpim | 0.031 | 0.014 | 0.356 |
| Total number of observations | 146995 | ||
| Number of reflections | 32133 | 210 | 1553 |
| <I/σ(I)> | 14.9 | 2.1 | |
| Completeness [%] | 99.2 | 91.3 | 98.7 |
| Redundancy | 4.6 | 4.8 | 3.7 |
| CC(1/2) | 0.999 | 0.999 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 22 % PEG 4000, 0.1 M Ammonium acetate, 0.1 M Sodium acetate pH 4.6 |
