6VDN

HCV NS3/4A protease A156T mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-21
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	54.111, 58.741, 60.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.690 - 2.150
R-factor	0.2008
Rwork	0.199
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p6m
RMSD bond length	0.007
RMSD bond angle	0.703
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.690	2.226
High resolution limit [Å]	2.149	2.149
Number of reflections	10927	1048
<I/σ(I)>	22.69
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.1M MES pH 6.5 2% Ammonium sulfate 20-25% PEG 3350