6VDA

Metal-bound C-terminal domain of CzcD transporter from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	H 3 2
Unit cell lengths	97.420, 97.420, 84.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.760 - 2.400
R-factor	0.23902
Rwork	0.234
R-free	0.28352
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zzt
RMSD bond length	0.005
RMSD bond angle	1.236
Data reduction software	XDS (5.8.0258)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (7.0.078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	6149	641
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	5.1
CC(1/2)	0.992	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6 M-2.0 M ammonium sulfate, 1.0 mM ZnSO4