6VD9

Metal-bound C-terminal domain of the CzcD transporter from Cuprividus metallidurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	32.760, 39.029, 63.965
Unit cell angles	98.78, 104.76, 90.09

Refinement procedure

Resolution	38.570 - 1.750
R-factor	0.2253
Rwork	0.224
R-free	0.25808
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vd8
RMSD bond length	0.010
RMSD bond angle	1.546
Data reduction software	XDS (5.8.0238)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (7.0.073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	29396	1610
<I/σ(I)>	7.2
Completeness [%]	96.3
Redundancy	2.9
CC(1/2)	0.990	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium sulphate, 0.1 M Bis-Tris pH 5.7, 22% (w/v) PEG 3350