6VD0
Crystal structure of Arabidopsis thaliana S-adenosylmethionine Synthase 2 (AtMAT2) in complex with free Methionine and AMPCPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-16 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.941, 84.721, 119.503 |
| Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
| Resolution | 39.830 - 2.000 |
| R-factor | 0.2226 |
| Rwork | 0.222 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vcz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.660 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.830 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.116 | 0.375 |
| Number of reflections | 136621 | 13511 |
| <I/σ(I)> | 8.78 | 2.13 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 3.21 | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.12 M Alcohols (1,6-hexanediol; 1-butanol; 1,2-propanediol; 2-propanol; 1,4-butanediol; 1,3-propanediol), 0.1 M HEPES and MOPS buffer at pH 7.5, 20% mmPEG500, 10% PEG 20000 |
